Investigating the involvement of proteins ATG24 (Tb927.9.13380) and Raptor N-terminal CASPase (Tb927.1.2190) in Trypanosoma brucei autophagy pathway.
Methods: crispr cas 9 endogenous , transfection , selection , western blot , Atuophagy induction , Immunofluorescence.
Hypothesis : ATG24 (Tb927.9.13380) and Raptor N-terminal CASPase (Tb927.1.2190) interact within the autophagy pathway of Trypanosoma brucei.
what specific roles do they play in the autophagy pathway ?
verify the localisation of ATG24 and RAPTOR genes
The aim of this project is to validate ATG24 (Tb927.9.13380) and Raptor N-terminal CASPase (Tb927.1.2190) proteins for their involvement in autophagy pathway.
I need a good introduction for this project .
ntroduction
The introduction should provide the context for the research question, identifying why the question is important and providing rationale for how it will be addressed. The aims and/or hypotheses should be clearly stated. You should utilize relevant primary research papers and summarize current understanding in the field. The introduction should not just be a general overview, rather it should start with the broader context and then focus in towards the specific project aims. It should articulate the research question being addressed, justifying the approach and outlining how your work will extend knowledge in this area. You should avoid describing methodological details (these should be in the methods). You should conclude your introduction with a clear, concise statement of aims.