Investigating the Protective Effects of M6 Peptide from Scorpion Venom on Stem Cell Renewal and Its Role in Mitigating Heavy Metal-Induced Oxidative Stress

Please prepare a progress report (not a final report) for the first semester of the school year, focusing on the following topic: Investigate the Protective Effects of M6 Peptide from Scorpion Venom on Stem Cell Renewal and Its Role in Mitigating Heavy Metal-Induced (specifically Cu2+) Oxidative Stress. 

 Here are the specifics to include: 
 Completed Work: 
  • Mention the MTT assay conducted to determine cell viability. 
  • Include the DCFH-DA fluorescent probe method used for ROS determination. 

Future Work: 

  • Outline the Western Blot assay planned for the next semester to determine the target protein. 

I have attached the following documents for your reference: 

  • A protocol detailing the entire experimentation procedure. 
  • A screenshot of the requirements for this progress report. 
  • Three sets of raw data: 
  1. An Excel file of the MTT assay raw data. 
  2. An Excel file of the ROS fluorescence intensity values. 
  3. A PowerPoint presentation containing fluorescence microscopic screenshots of the GFP. 

Please feel free to reach out if you have any questions. Thank you!

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